July 2025 – Optimal Expression System – Part 1 – Structural Complexity
7/10/2025
Choosing the Right Expression System Understanding the structural complexity of your protein of interest is a foundational step in selecting the optimal expression system for recombinant protein production. This choice directly impacts the success of your project, influencing protein yield, quality, and functionality. Let’s explore how structural complexity guides the selection process. Proteins with simple…
Part 5 – Recombinant Antigens, Methods for Determining Protein Quality
5/27/2025
Ensuring Excellence: Methods for Assessing Recombinant Antigen Quality The production of recombinant antigens involves numerous considerations, such as construct design, affinity tags, expression system, purification scheme, formulation, and characterization. Among these, rigorous quality assessment is vital to ensure the antigen’s suitability for downstream applications. Below, we highlight key methods to evaluate recombinant antigen quality prior…
Maximizing Impact: Long-term Storage of Recombinant Antigens
4/28/2025
Producing recombinant proteins, particularly recombinant antigens, involves a multitude of variables. These include construct design, affinity tags, expression system, purification scheme, formulation, and characterization. Among these, long-term storage considerations play a pivotal role in preserving protein quality and functionality. The Importance of Buffer Formulation Buffer formulation is critical for long-term storage, especially for recombinant…
Best Practices: Generating Multiple Expression Constructs
7/23/2024
Generating multiple protein constructs through alteration of affinity tag selection, size, and placement can be highly beneficial for projects with no prior precedent of successful recombinant protein production.
