E. coli Expression

Proteos provides expression of recombinant proteins in E.coli for rapid, economical production. This system can be used for proteins of prokaryotic origin or eukaryotic origin if complex post-translation modifications are not required for proper folding and function.

Our expression platform utilizes numerous E. coli strains and fully validated expression protocols for consistent and reproducible protein expression in shake flask culture volumes at up to 24 L batch size.

Typical productions times are 1-2 weeks for production of deliverable cell paste, 2-4 weeks for purified protein production starting from an expression plasmid or glycerol stock, or 6-8 weeks for purified protein production starting from construct design.   

Services offered:

  • Full suite of molecular biology services to accompany any of our expression platforms, including construct design, fusion partner and affinity tag selection, gene synthesis with codon optimization, subcloning, endotoxin-free plasmid DNA isolation, and sequence verification.
  • Expression verification and optimization at a 50 mL culture scale; ideal for expression testing of multiple recombinant protein targets or expression conditions in parallel.  Optimization parameters may include selection of E. coli host strain, media, induction conditions, incubation time, and/or incubation temperature. 
  • Pilot scale production and yield determination at culture scales ranging from 2 L to 4 L; ideal for process verification through purification prior to large-scale production.
  • Preparative scale production of recombinant proteins in shake flask culture volumes up to a 40 L batch size.
  • Generation and banking of glycerol stocks for rapid scale-up and expression in future production runs.
  • High-throughput expression and purification of up to 96 His-tagged protein constructs per production run at a 15 mL culture scale.
  • E.coli expression services can be combined with Proteos’ purification services for a fully-customized production process.

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