Unlocking the Secrets of Protein Complexity

Choosing the Right Expression System

Understanding the structural complexity of your protein of interest is a foundational step in selecting the optimal expression system for recombinant protein production. This choice directly impacts the success of your project, influencing protein yield, quality, and functionality. Let’s explore how structural complexity guides the selection process. 

Proteins with simple structures, such as single-domain prokaryotic proteins, are ideal candidates for bacterial expression systems. Bacteria offer a streamlined process with rapid growth and economic scalability. However, when dealing with eukaryotic proteins that have complex tertiary or quaternary structures, bacterial systems often fall short. These proteins require the advanced folding machinery present in insect or mammalian cells to achieve their native conformation. 

Choosing the wrong system can lead to misfolded or biologically inactive proteins. By aligning the expression system with the structural demands of the target protein, researchers can maximize both efficiency and functionality. 

Key Takeaways: 

  • Bacteria (E. coli) may be a useful and economical solution for recombinant proteins with simple structures. 
  • Avoid misfolding by choosing an expression system that supports native protein conformation. 

Proteos will work with you to identify and implement the optimal expression for your project needs. Contact us to learn more about our customizable solutions for recombinant protein production. 

Graphic of multi-colored protein structure | proteos | protein production CRO