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Protein Analytics

Colorful infographic depicting customized protein analytics services from Proteos | US-based recombinant protein production CRO

Comprehensive Protein Analytics Services

Customizable protein analytics packages to meet your specific needs.

Intact Mass Analysis (PDF)
Thermal Shift Analysis (TSA) (PDF)
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Our Protein Analytics platform offers a full suite of methods to characterize, quantify, and evaluate protein samples across multiple dimensions. Analytics packages are fully customizable to best suit your applications. Below is an overview of the key analytical techniques we offer.

 

Protein Separation and Detection Techniques 

SDS-PAGE and Western Blotting  

High-resolution protein separation and immunodetection for molecular weight estimation and confirmation of protein identity. 

Protein Concentration and Quantification 

Colorimetric Protein Assays 

Sensitive detection using BCA, Bradford, or other methods for accurate protein concentration measurements. 

Protein Quantitation via NanoDrop 

Rapid, small-volume protein concentration analysis based on absorbance at 280 nm. 

Protein Purity and Structural Analysis 

Analytical HPLC-SEC 

Size-Exclusion Chromatography (SEC) for protein purity, aggregation, and oligomerization state determination. 

Melting Temperature Determination via Thermal Shift Assay 

Evaluate protein thermal stability and binding interactions through differential scanning fluorimetry (DSF) 

Click here for additional information about Thermal Shift Assay analysis.

 

Enzymatic and Functional Assays 

Qualitative Colorimetric and Fluorometric Enzyme Assays 

Assess enzymatic activity using commercially available kits to measure substrate conversion with visible or fluorescent signal readout.  

Contaminant and Impurity Testing 

Endotoxin Determination via LAL Assay 

Quantitative detection of endotoxin levels using the Charles River’s Endosafe® PTS system that utilizes Limulus Amebocyte Lysate (LAL) for rapid endotoxin testing. The Endosafe® PTS™ is FDA-licensed and meets the requirements of USP<85> and Ph. Eur. <2.6.14> for LAL testing.   

Mass Spectrometry-Based Characterization 

Intact Mass Analysis 

Rapid and reliable intact mass analysis of proteins using the Waters BioAccord LC-MS system. Click here for additional information about Intact Mass Analysis. 

Frequently Asked Questions

Do you test the functionality of the recombinant proteins you produce, and can you confirm they will work in my application?

We perform standard analytical testing on all recombinant proteins but cannot guarantee that a protein will be functional in your specific experimental system. Functional performance may vary depending on the application, formulation, assay conditions, and any downstream modifications. 

What is typically included in a standard analytics package?

A standard protein analytics package includes:

  • SDS-PAGE: Reducing and non-reducing to assess disulfide bond formation 
  • Analytical HPLC-SEC: Pre- and Post- freeze/thaw  
  • Protein Quantitation: Absorbance at A280 measured on a NanoDrop  
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