Protein Analytics
Proteos offers a suite of protein analytics for analysis of your protein samples or as a complement to our recombinant protein production services.
- SDS-PAGE/western blotting
- Colorimetric protein assays (Bradford, BCA, microBCA, 660nm)
- Protein quantitation via Nanodrop
- Analytical HPLC-SEC
- Intact mass analysis
- Colorimetric and fluorometric enzyme assays
- Endotoxin determination via LAL assay
- Protein melting temperature determination via thermal shift assay which is useful for batch to batch validation, buffer screening for formulation optimization, ligand binding studies, protein:protein binding studies, and construct stability comparisons
Learn more about thermal shift assay
Analytical HPLC-SEC data with experimentally determined molecular weights of a Fab (red trace) and full length IgG (blue trace). Molecular weights were experimentally determined using a calibration curve assemble from a set of 5 standards shown in the lower chromatogram (black trace). Standards include thyroglobulin (670,000 Da), δ-globulin (158,000 Da), ovalbumin (44,000 Da), myoglobin (17,000 Da) and vitamin B12 (1,350 Da).