Recombinant Protein Production Services
Proteos offers a wide range of recombinant protein and antibody expression platforms, purification methods, analysis tools, and assay services, which enable us to provide complete production from concept to reagent. Each service can be customized to meet your individual project specifications.
Antibody Production
Recombinant expression and purification of full-length IgG antibody, F(ab) and (Fab’)2 antibody fragments, and antibody fusion proteins. Our antibody production capabilities include construct design, transient mammalian cell expression, utilizing in-house or customer provided expression vectors, and affinity purification. We offer both molecular and enzymatic approaches for generation of F(ab) and (Fab’)2 antibody fragments.
Protein Expression
Mammalian cell, insect cell, and bacterial expression platforms are available for expression of recombinant proteins. Complete molecular biology services, from construct design to endotoxin free plasmid DNA isolation, are also available.
Protein Purification
A comprehensive selection of protein purification and analysis services for isolation and characterization of recombinant affinity-tagged or native proteins are available. In addition, we also offer customizable protein analytics packages.
Protein Assay Services
The baculovirus titer assay is a plate-based flow cytometry assay, that offers a quick reproducible method for determining the number of infectious virus particles/mL.
The thermal shift assay (TSA) is used to measure the thermal denaturation (melting temperature) of proteins under various conditions. The assay is useful for screening pH, ionic strength, ligand binding, and other formulation additives for their impact on melting temperature. Conditions that increase the melting temperature are considered stabilizing.
We also offer services for running commercially available kit-based colorimetric and fluorometric assays.