Whitepaper: Cryo-EM Structure of CYP3A4

Successful cryo-EM structure determination depends on high-quality protein samples. This whitepaper describes a gene-to-structure case study for CYP3A4, a 57 kDa cytochrome P450 monooxygenase.  

CYP3A4 is the most extensively studied P450 enzyme due to its role in drug metabolism. This structure data may have a significant impact on drug development and toxicology studies.  

Recombinant CYP3A4 was produced by Proteos with structure determination completed at NanoImaging Services (NIS). 

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Colorful graphic of cryo-EM structure of soluble protein CYP3A4, produced by Protes