Maximizing Impact: Long-term Storage of Recombinant Antigens

Producing recombinant proteins, particularly recombinant antigens, involves a multitude of variables. These include construct design, affinity tags, expression system, purification scheme, formulation, and characterization. Among these, long-term storage considerations play a pivotal role in preserving protein quality and functionality.

The Importance of Buffer Formulation

Buffer formulation is critical for long-term storage, especially for recombinant antigens intended for in vivo antibody production. The immunization buffer should closely mimic physiological conditions to ensure compatibility and effectiveness. Additives, such as detergents and reducing agents, must be kept at low concentrations to mitigate in vivo toxicity risks.

Tailoring the protein concentration and aliquot size to the specific immunization protocol is equally important. These adjustments minimize unnecessary freeze-thaw cycles, ensuring the recombinant antigen is immediately usable upon thawing. By doing so, you maximize the efficiency of antibody production during immunization and preserve protein integrity over extended periods.

Custom Solutions with Proteos

At Proteos, we specialize in tailoring every project to meet your unique research needs. Our workflows are meticulously designed to ensure your protein deliverables are optimized for formulation, concentration, aliquot volume, and storage temperature. Whether your goal is in vivo antibody production or another application, our expertise ensures your recombinant antigens are delivered ready for use.

As an industry leader in high-quality recombinant protein antigen production for drug discovery research, Proteos is committed to your success. Contact us today to discuss your specific project requirements and explore how we can support your research goals.