Recombinant Antigens: Affinity Tags

Proteos Recombinant Protein Expression and Purification
6/23/2021

There are many variables to consider when producing recombinant proteins, and more specifically recombinant antigens.  These variables include construct design, affinity tags, expression system, purification scheme, formulation, and characterization.  Here we will focus on the use of affinity tags in recombinant antigen production.

When designing a recombinant antigen expression construct and purification workflow, it is worth considering an affinity tag. Adding an affinity tag to a recombinant antigen can simplify purification and downstream assays. However, affinity tags can complicate immunizations for antibody production. For example, affinity tags can alter the structure of the antigen, rendering certain epitopes inaccessible to B cell recognition. This reduces the immunogenicity of the antigen, leading to reduced antibody production. In addition, the tags themselves can trigger an immune response. This means that the resulting preparation will be a mixture of antibodies against the tag and the intended target antigen. Larger affinity tags may cause more issues than smaller tags, but this is not guaranteed. To avoid complications during antibody production, the constructs can be designed such that the affinity tag can be removed via protease digestion during the purification process.

Previous blog posts have discussed different aspects of affinity tag use in more depth.

At Proteos, our experienced scientists understand that each recombinant protein production project is unique and will work with you to develop a fit-for-purpose product from our initial conversation.

Contact us today.  We will guide you through the process and design a custom quotation that fits your specific project needs.

Tags: Affinity tags, expression, purification, Recombinant antigen production

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