Fusion Proteins for Prokaryotic Expression
Proteos is a reputable contract service organization, committed to providing high-quality recombinant protein production services that meet or exceed client expectations. With decades of experience, the scientific staff can anticipate challenges that may arise during protein production and leverage their knowledge to devise innovative solutions.
Protein aggregation is a common issue when producing a eukaryotic protein in a prokaryotic expression system. Prokaryotes lack complex chaperone pathways, so expression of a eukaryotic construct may result in a misfolded product that is sequestered to inclusion bodies.
Fusion proteins, such as MBP and GST, are affinity tags used to improve the solubility of recombinant proteins produced in prokaryotic cells. The tags themselves fold quickly into stable conformations after ribosomal translation, and these traits are often shared with the recombinant protein to which they are attached. During purification, the recombinant protein can be captured with an affinity chromatography resin that binds to the fusion protein.
Upcoming blogs will discuss fusion protein selection, placement, and removal.
The Proteos team can take your project from inception to protein. We provide customized, adaptable, milestone-driven scopes of work to facilitate data-driven decision making while mitigating risks. Contact us today to start the discussion.