Selecting a Eukaryotic Signal Sequence
Proteos is a reputable contract service organization, committed to providing high-quality recombinant protein production services and deliverables that meet or exceed client expectations. The scientific staff has decades of experience. They can anticipate challenges that may arise during protein production and leverage their knowledge and past successes to devise innovative solutions and optimize the overall workflow.
Selection of the proper signal sequence for recombinant protein production in mammalian and insect cell culture is a crucial step that should not be overlooked during construct design.
If the protein of interest contains a native signal sequence, this can be retained in the expression construct design. Many signal sequences are functional in heterologous organisms, which is convenient for recombinant production. If recombinant expression and/or secretion is low with the native sequence, it can be replaced with a heterologous signal sequence. For example, a signal sequence that is native to the insect or mammalian expression host cell line may improve recombinant production of the protein of interest. It is wise to design multiple constructs with native and heterologous signal sequences to determine empirically which one works best.
The Proteos team can take your project from inception to protein. We provide customized, adaptable, milestone-driven scopes of work to facilitate data-driven decision making while mitigating risks. Contact us today to start the discussion.