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Custom Protein Production

Proteos specializes in custom recombinant protein production.  All of our services are fully customizable and can be ordered individually or as part of a complete protein production project.  For custom protein production, please request a quotation through the link below.  For questions regarding custom services, please use the Contact Us form.

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Assay Services

Our assay services are listed below for easy ordering or may be requested by using the Contact Us form.

Recombinant Protein Products

Recombinant proteins produced in collaboration with the MJFF are listed below for easy ordering or may be requested by using the Contact Us form. 

Assay Services

SKU
Title
Quantity
Price
Purchase
SKUAS-001
TitleBaculovirus titer assay (single sample)
Quantity
Price$320.00
Description

Flow cytometry assay for baculovirus titer determinations.  Results are reported in infectious units per milliliter (IU/mL) of baculovirus stock solution.

Deliverables

Data sheet detailing titer data.

Required Materials & Information

A minimum of 600 microliters of stock is required for each submitted sample (1 mL preferred).

Additional Notes

This price is for a single sample.  Please use AS-007 for ordering multiple samples.

SKUAS-007
TitleBaculovirus titer assay (multiple samples)
Quantity
Price$160.00
Description

Flow cytometry assay for baculovirus titer determinations.  Results are reported in infectious units per milliliter (IU/mL) of baculovirus stock solution.

Deliverables

Data sheet detailing titer data.

Required Materials & Information

A minimum of 600 microliters of stock is required for each submitted sample (1 mL preferred).

Additional Notes

This price is for multiple samples submitted at the same time.  Single samples will need to be ordered using item AS-001.

SKUAS-002
TitleTSA Tm determination (per sample)
Quantity
Price$265.00
Description

Thermal shift analysis (TSA).  Melting temperature deterimation (triplicate analysis, single formulation).

Deliverables

Data sheet detailing assay results.

Required Materials & Information

0.2 mL of each protein sample at a concentration of 1 mg/mL or greater.

SKUAS-003
TitleTSA standard buffer screen (per plate, single protein)
Quantity
Price$1,010.00
Description

Thermal shift analysis (TSA). Thermal denaturation assay to screen buffer conditions (pH, salt, additives).  Conditions that cause the Tm to shift to a higher temperature are identified as stabilizing.

Deliverables

Data sheet detailing assay results.

Required Materials & Information

2 mL of protein sample at a concentration of 1 mg/mL or greater.

SKUAS-004
TitleTSA ligand binding screen (per plate)
Quantity
Price$1,010.00
Description

Thermal shift analysis (TSA). Thermal denaturation assay to assess ligand:protein binding.  Compounds that cause the Tm of the protein to shift are flagged as target protein ligands.

Deliverables

Data sheet detailing assay results.

Required Materials & Information

2 mL of each protein sample at a concentration of 1 mg/mL or greater.

SKUAS-005
TitleTSA protein:protein binding study (per plate)
Quantity
Price$1,010.00
Description

Thermal shift analysis (TSA). Thermal denaturation assay to assess protein:protein binding.  Tm shifts and denaturation curve changes are indicative of protein:protein interactions.

Deliverables

Data sheet detailing assay results.

Required Materials & Information

2 mL of each protein sample at a concentration of 1 mg/mL or greater.

SKUAS-006
TitleConstruct stability comparison (per plate)
Quantity
Price$1,010.00
Description

Thermal shift analysis (TSA). Thermal denaturation assay to assess changes in Tm among a set of protein variants.  Protein variants with higher Tm are identified as more stable.

Deliverables

Data sheet detailing assay results

Required Materials & Information

2 mL of each protein sample at a concentration of 1 mg/mL or greater.

Recombinant Protein Products

SKU
Title
Quantity
Price
Purchase
SKURP-002
TitleHuman Alpha-synuclein filament - Proteos Discontinued - Available from Abcam (see details for link)
PriceS&H
Description

Product #RP-002 Human Alpha Synuclein Filaments has been discontinued by Proteos.  This product is now available from Abcam (product #Ab254309) at 

MJFF Research Tools Catalog

Full-Length human alpha-synuclein protein expressed in vitro and manipulated to form alpha-synuclein filaments. This filament version of alpha-synuclein was used as antigen for the MJF-14 conformation-specific rabbit monoclonal alpha-synuclein filament specific antibody available from Abcam (ab209538).  

Deliverables
Required Materials & Information
SKURP-003
TitleHuman Alpha-synuclein monomer to generate preformed fibrils
Quantity
Price$250.00
Description

Full-Length human alpha-synuclein monomeric protein specially formulated to generate pre-formed fibrils.  Shipped on dry ice, store at -80 °C upon arrival. Avoid freeze/thaw cycles.

Polinski NK, Volpicelli-Daley LA, Sortwell CE, et al. Best Practices for Generating and Using Alpha-Synuclein Pre-Formed Fibrils to Model Parkinson’s Disease in Rodents. Journal of Parkinson’s disease. 2018;8(2):303-322. doi:10.3233/JPD-171248.  

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004926/pdf/jpd-8-jpd171248.pdf

JoVE video article on how to generate, handle, and use aSyn PFFs.

Deliverables

Protein is supplied in 1 mg aliquots with a COA

Required Materials & Information

Agree to terms and conditions upon checkout.

Additional Notes

10 x 1  mg aliquot maximum/order

SKURP-001
TitleHuman Alpha-synuclein monomer (1 mg)
Quantity
Price$250.00
Description

Full-Length human alpha-synuclein monomeric protein generated in vitro. This protein was used to generate alpha-synuclein filament which was then used as antigen for the MJF-14 alpha-synuclein filament-specific antibody available from Abcam (ab209538).  Shipped at ambient temperature, store at -80 °C upon arrival.

Deliverables

Protein is supplied in 1 mg aliquots with a COA

Required Materials & Information

Agree to terms and conditions upon checkout.

Additional Notes

10 x 1  mg aliquot maximum/order

SKURP-004
TitleHuman Alpha-synuclein phospho-Ser129 peptide
Quantity
Price$325.00
Description

Phospho-S129 Alpha Synuclein semi-synthetic phosphomimetic peptide generated by native chemical ligation of a recombinant peptide produced from E. coli and a synthetic peptide.  Shipped at ambient temperature, store at -20 °C upon arrival. Store at -80 °C in single use aliquots after reconstitution.

Deliverables

Protein is supplied in 1 mg aliquots with a COA

Required Materials & Information

Agree to terms and conditions upon checkout.

Additional Notes

10 x 1  mg aliquot maximum/order

SKURP-009
TitleMouse Alpha-synuclein monomer to generate preformed fibrils
Quantity
Price$250.00
Description

Full-Length mouse alpha-synuclein monomeric protein specially formulated to generate pre-formed fibrils. Product is recommended for in vitro use as in vivo toxicity has not been reported with this protein. Shipped on dry ice, store at -80 °C upon arrival. Avoid freeze/thaw cycles. 

Polinski NK, Volpicelli-Daley LA, Sortwell CE, et al. Best Practices for Generating and Using Alpha-Synuclein Pre-Formed Fibrils to Model Parkinson’s Disease in Rodents. Journal of Parkinson’s disease. 2018;8(2):303-322. doi:10.3233/JPD-171248. 

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004926/pdf/jpd-8-jpd171248.pdf

JoVE video article on how to generate, handle, and use aSyn PFFs.

Deliverables

Protein is supplied in 1 mg aliquots with a COA

Required Materials & Information

Agree to terms and conditions upon checkout.

Additional Notes

10 x 1  mg aliquot maximum/order

SKURP-005
TitleDJ-1 (Untagged, E. coli)
Quantity
PriceS&H
Description

Recombinant untagged human DJ-1 protein produced in E. coli. 

Deliverables

Protein is supplied in 1 mg aliquots with a COA

Required Materials & Information

Agree to terms and conditions upon checkout.

Additional Notes

2 x 1  mg aliquot maximum/order

SKURP-006
TitleDJ-1 (His-tagged, E. coli)
Quantity
PriceS&H
Description

Recombinant His-tagged human DJ-1 protein produced in E. coli.  

Deliverables

Protein is supplied in 1 mg aliquots with a COA

Required Materials & Information

Agree to terms and conditions upon checkout.

Additional Notes

2 x 1  mg aliquot maximum/order

SKURP-007
TitleDJ-1 (His-tagged, mammalian)
Quantity
PriceS&H
Description

Recombinant His-tagged DJ-1 protein produced in a mammalian cell expression system (HEK).

Deliverables

Protein is supplied in 1.6 mg aliquots with COA

Required Materials & Information

Agree to terms and conditions upon checkout.

Additional Notes

2 x 1  mg aliquot maximum/order

SKURP-008
TitleDJ-1 (TEV-cleaved, mammalian)
Quantity
PriceS&H
Description

Recombinant TEV-cleaved (to remove His-tag) DJ-1 protein produced in a mammalian cell expression system (HEK).

Deliverables

Protein is supplied in 1.05 mg aliquots with a data COA

Required Materials & Information

Agree to terms and conditions upon checkout.

Additional Notes

2 x 1  mg aliquot maximum/order

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