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Proteos specializes in custom recombinant protein production.  All of our services are fully customizable and can be ordered individually or as part of a complete protein production project.  For custom protein production, please request a quotation through the link below.  For questions regarding custom services, please contact us through this link.

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Assay services, protein production packages, and recombinant protein products are listed below for easy ordering.  The protein production packages have been predefined to give our customers a streamlined alternative to custom production services.  When ordering via the website, please be sure to check your order for accuracy and ensure the appropriate add-ons are included with the protein production packages.  Once your order is placed, our customer service team will communicate our progress throughout the project.   We look forward to working with you!

For all questions regarding catalog items, you can contact us at customerservice@proteos.com or visit our FAQs page.

Frequently Asked Questions

Assay Services

SKU
Title
Quantity
Price
Purchase
SKUAS-001
TitleBaculovirus titer assay
Quantity
Price$150.00
Description

Flow cytometry assay for baculovirus titer determinations.  Results are reported in infectious units per milliliter (IU/mL) of baculovirus stock solution.

Deliverables

Data sheet detailing titer data for each sample.

Required Materials & Information

A minimum of 600 microliters of stock is required for each submitted sample.

Additional Notes

Maximum 10 samples per order.  Custom pricing available for orders greater than 10 samples

SKUAS-002
TitleTSA Tm determination (single sample)
Quantity
Price$200.00
Description

Thermal shift analysis (TSA).  Melting temperature deterimation (triplicates analysis, single formulation)

Deliverables

Data sheet detailing assay results.

Required Materials & Information

0.2 mL of each protein sample at a concentration of 1 mg/mL or greater.

SKUAS-003
TitleTSA standard buffer screen (single sample)
Quantity
Price$500.00
Description

Thermal shift analysis (TSA). Thermal denaturation assay to screen buffer conditions (pH, salt, additives).  Conditions that cause the Tm to shift to a higher temperature are identified as stabilizing.

Deliverables

Data sheet detailing assay results.

Required Materials & Information

2 mL of each protein sample at a concentration of 1 mg/mL or greater.

SKUAS-004
TitleTSA ligand binding screen (single sample)
Quantity
Price$1,000.00
Description

Thermal shift analysis (TSA). Thermal denaturation assay to assess ligand:protein binding.  Compounds that cause the Tm of the protein to shift are flagged as target protein ligands.

Deliverables

Data sheet detailing assay results.

Required Materials & Information

2 mL of each protein sample at a concentration of 1 mg/mL or greater.

SKUAS-005
TitleTSA protein:protein binding study (single sample)
Quantity
Price$1,000.00
Description

Thermal shift analysis (TSA). Thermal denaturation assay to assess protein:protein binding.  Tm shifts and denaturation curve changes are indicative of protein:protein interactions.

Deliverables

Data sheet detailing assay results.

Required Materials & Information

2 mL of each protein sample at a concentration of 1 mg/mL or greater.

SKUAS-006
TitleConstruct stability comparison
Quantity
Price$1,000.00
Description

Thermal shift analysis (TSA). Thermal denaturation assay to assess changes in Tm among a set of protein variants.  Protein variants with higher Tm are identified as more stable.

Deliverables

Data sheet detailing assay results

Required Materials & Information

2 mL of each protein sample at a concentration of 1 mg/mL or greater.

Protein Production Packages

SKU
Title
Quantity
Price
Purchase
SKUPP-001
TitleSecreted His-tagged protein production in HEK cells, 4 L scale
Quantity
Price$6,600.00
Addons

Base Pairs: +$( BasePairs * .40 )
Description

Production of synthetic cDNA with codon optimization for expression in E. coli, baculovirus, or mammalian cell (HEK293) expression systems. Subcloning of synthetic cDNA into appropriate expression or transfer vector for protein production.

Deliverables

Expression or transfer vector banked at Proteos for 1 year

Additional Notes

Vectors are not transferrable due to liscensing restrictions

Description

Endotoxin-free plasmid preparation at the giga-prep scale for mammalian cell expression (typically yields 4 mg of DNA)

Additional Notes

Can be added to HEK293 protein production packages. Must be high-copy number plasmid, must order one prep each for heavy chain and light chain if not using a dual expression vector

Description

Analytical size exclusion chromatography of purified protein to assess purity and monodispersity

Description

Size exclusion chromatography (gel filtration) following Protein A purification, 1.6x60 or 2.6x60 cm column based on expression analysis estimate, destination buffer of choice

Additional Notes

TBS destination buffer (PBS for Mab or Fc) will be used if no destination buffer is specified

Request Quote

Description

Transient expression of a secreted His-tagged protein in HEK293 cells at the 4 L culture scale.  Single step Ni-NTA affinity purification, dialysis into into destination buffer, concentration targeting 1 mg/mL. Delivered frozen on dry ice in 0.5 mL aliquots.

Deliverables

All purified protein and a data sheet with final results.

Required Materials & Information

4 mg of endotoxin-free mammalian cell expression plasmid DNA encoding a SECRETED His-tagged protein of interest, destination buffer formulation

Additional Notes

Secreted protein expression only, TBS based destination buffer will be used if no destination buffer is specified, protein concentration will be terminated if precipitation is observed.

SKUPP-002
TitleHis-tagged protein production in E. coli, 4 L scale
Quantity
Price$5,100.00
Addons

Base Pairs: +$( BasePairs * .40 )
Description

Production of synthetic cDNA with codon optimization for expression in E. coli, baculovirus, or mammalian cell (HEK293) expression systems. Subcloning of synthetic cDNA into appropriate expression or transfer vector for protein production.

Deliverables

Expression or transfer vector banked at Proteos for 1 year

Additional Notes

Vectors are not transferrable due to liscensing restrictions

Description

Analytical size exclusion chromatography of purified protein to assess purity and monodispersity

Description

Size exclusion chromatography (gel filtration) following Protein A purification, 1.6x60 or 2.6x60 cm column based on expression analysis estimate, destination buffer of choice

Additional Notes

TBS destination buffer (PBS for Mab or Fc) will be used if no destination buffer is specified

Request Quote

Description

E. coli culture expanded to the 4 L scale, expression induced at 0.6 OD with 1 mM IPTG, harvested 4 hours post induction. Single step Ni-NTA affinity purification, dialysis into into destination buffer, concentration targeting 1 mg/mL. Delivered frozen on dry ice in 0.5 mL aliquots.

Deliverables

All purified protein and a data sheet with final results.

Required Materials & Information

IPTG inducible expression plasmid  encoding His-tagged protein of interest or glycerol stock, antibiotic resistance information, destination buffer formulation

Additional Notes

TBS based destination buffer will be used if no destination buffer is specified, protein concentration will be terminated if precipitation is observed

SKUPP-003
TitleSecreted His-tagged protein production, in Baculovirus infected Tni PRO cells, 2 L scale
Quantity
Price$8,400.00
Addons

Base Pairs: +$( BasePairs * .40 )
Description

Production of synthetic cDNA with codon optimization for expression in E. coli, baculovirus, or mammalian cell (HEK293) expression systems. Subcloning of synthetic cDNA into appropriate expression or transfer vector for protein production.

Deliverables

Expression or transfer vector banked at Proteos for 1 year

Additional Notes

Vectors are not transferrable due to liscensing restrictions

Description

Analytical size exclusion chromatography of purified protein to assess purity and monodispersity

Description

Size exclusion chromatography (gel filtration) following Protein A purification, 1.6x60 or 2.6x60 cm column based on expression analysis estimate, destination buffer of choice

Additional Notes

TBS destination buffer (PBS for Mab or Fc) will be used if no destination buffer is specified

Request Quote

Description

Insect cells infected with high-titer baculovirus stock at a MOI=3, harvested 24 hours post infection, 5 vials cryopreserved at 1 x 10^7 cells/mL, stored in liquid nitrogen vapor phase

Deliverables

BIIC stocks delivered or stored at Proteos

Description

Baculovirus mediated expression of a secreted His-tagged protein in Tni PRO insect cells at the  2 L culture scale. Diafiltration of conditioned culture supernatant into a Ni-NTA compatible buffer. Single step Ni-NTA affinity purification, dialysis into destination buffer, concentration targeting 1 mg/mL. Delivered frozen on dry ice in 0.5 mL aliquots.

Deliverables

All purified protein and a data sheet with final results.

Required Materials & Information

Baculovirus transfer vector encoding a SECRETED His-tagged protein of interest, preferred MOI, destination buffer formulation

Additional Notes

Includes generation of high-titer baculovirus stock for infection, secreted protein expression only, TBS based destination buffer will be used if no destination buffer is specified, protein concentration will be terminated if precipitation is observed

SKUPP-004
TitlemAb or Fc-fusion protein production in HEK293 cells, 4 L scale
Quantity
Price$6,800.00
Addons

Base Pairs: +$( BasePairs * .40 )
Description

Production of synthetic cDNA with codon optimization for expression in E. coli, baculovirus, or mammalian cell (HEK293) expression systems. Subcloning of synthetic cDNA into appropriate expression or transfer vector for protein production.

Deliverables

Expression or transfer vector banked at Proteos for 1 year

Additional Notes

Vectors are not transferrable due to liscensing restrictions

Description

Endotoxin-free plasmid preparation at the giga-prep scale for mammalian cell expression (typically yields 4 mg of DNA)

Additional Notes

Can be added to HEK293 protein production packages. Must be high-copy number plasmid, must order one prep each for heavy chain and light chain if not using a dual expression vector

Description

Analytical size exclusion chromatography of purified protein to assess purity and monodispersity

Description

Size exclusion chromatography (gel filtration) following Protein A purification, 1.6x60 or 2.6x60 cm column based on expression analysis estimate, destination buffer of choice

Additional Notes

TBS destination buffer (PBS for Mab or Fc) will be used if no destination buffer is specified

Request Quote

Description

Transient expression of mAb or Fc fusion protein in HEK293 cells at the 4 L culture scale.  Single step Protein A affinity purification, dialysis into into destination buffer,  concentration targeting 1 mg/mL. mAb delivered in a single tube on wet ice, Fc-fusion protein delivered frozen on dry ice in 0.5 mL aliquots.

Deliverables

All purified protein and a data sheet with final results.

Required Materials & Information

4 mg of endotoxin-free expression plasmid DNA, destination buffer formulation

Additional Notes

2 mg of each expression plasmid DNA encoding the heavy chain and light chain will be required if not  using a dual vector system, PBS will be used as destination buffer if no destination buffer is specified, maximum resin bed for Protein A will be 40 mL with a maximum of 2 passes

SKUPP-005
TitleSecreted His-tagged protein production in HEK cells, 20 L scale,
Quantity
Price$14,600.00
Addons

Base Pairs: +$( BasePairs * .40 )
Description

Production of synthetic cDNA with codon optimization for expression in E. coli, baculovirus, or mammalian cell (HEK293) expression systems. Subcloning of synthetic cDNA into appropriate expression or transfer vector for protein production.

Deliverables

Expression or transfer vector banked at Proteos for 1 year

Additional Notes

Vectors are not transferrable due to liscensing restrictions

Description

Endotoxin-free plasmid preparation at the giga-prep scale for mammalian cell expression (typically yields 10 mg of DNA)

Additional Notes

Can be added to HEK293 protein production packages. Must be high-copy number plasmid, must order one prep each for heavy chain and light chain if not using a dual expression vector

Description

Analytical size exclusion chromatography of purified protein to assess purity and monodispersity

Description

Size exclusion chromatography (gel filtration) following Protein A purification, 1.6x60 or 2.6x60 cm column based on expression analysis estimate, destination buffer of choice

Additional Notes

TBS destination buffer (PBS for Mab or Fc) will be used if no destination buffer is specified

Request Quote

Description

Transient expression of a secreted His-tagged protein in HEK293 cells at the 20 L culture scale.  Single step Ni-NTA affinity purification, dialysis into into destination buffer, concentration targeting 1 mg/mL. Delivered frozen on dry ice in 0.5 mL aliquots.

Deliverables

All purified protein and a data sheet with final results.

Required Materials & Information

20 mg of endotoxin-free mammalian cell expression plasmid DNA encoding a SECRETED His-tagged protein of interest, destination buffer formulation

Additional Notes

Secreted protein expression only, TBS based destination buffer will be used if no destination buffer is specified, protein concentration will be terminated if precipitation is observed

SKUPP-006
TitleHis-tagged protein production in E. coli, 20 L scale
Quantity
Price$8,900.00
Addons

Base Pairs: +$( BasePairs * .40 )
Description

Production of synthetic cDNA with codon optimization for expression in E. coli, baculovirus, or mammalian cell (HEK293) expression systems. Subcloning of synthetic cDNA into appropriate expression or transfer vector for protein production.

Deliverables

Expression or transfer vector banked at Proteos for 1 year

Additional Notes

Vectors are not transferrable due to liscensing restrictions

Description

Analytical size exclusion chromatography of purified protein to assess purity and monodispersity

Description

Size exclusion chromatography (gel filtration) following Protein A purification, 1.6x60 or 2.6x60 cm column based on expression analysis estimate, destination buffer of choice

Additional Notes

TBS destination buffer (PBS for Mab or Fc) will be used if no destination buffer is specified

Request Quote

Description

E. coli culture expanded to the 20 L scale, expression induced at 0.6 OD with 1 mM IPTG, harvested 4 hours post induction. Single step Ni-NTA affinity purification, dialysis into into destination buffer, concentration targeting 1 mg/mL. Delivered frozen on dry ice in 0.5 mL aliquots.

Deliverables

All purified protein and a data sheet with final results.

Required Materials & Information

IPTG inducible expression plasmid  encoding His-tagged protein of interest or glycerol stock, antibiotic resistance information, destination buffer formulation

Additional Notes

TBS based destination buffer will be used if no destination buffer is specified, protein concentration will be terminated if precipitation is observed

SKUPP-007
TitleSecreted His-tagged protein production, in Baculovirus infected Tni PRO cells, 20 L scale
Quantity
Price$14,300.00
Addons

Base Pairs: +$( BasePairs * .40 )
Description

Production of synthetic cDNA with codon optimization for expression in E. coli, baculovirus, or mammalian cell (HEK293) expression systems. Subcloning of synthetic cDNA into appropriate expression or transfer vector for protein production.

Deliverables

Expression or transfer vector banked at Proteos for 1 year

Additional Notes

Vectors are not transferrable due to liscensing restrictions

Description

Analytical size exclusion chromatography of purified protein to assess purity and monodispersity

Description

Size exclusion chromatography (gel filtration) following Protein A purification, 1.6x60 or 2.6x60 cm column based on expression analysis estimate, destination buffer of choice

Additional Notes

TBS destination buffer (PBS for Mab or Fc) will be used if no destination buffer is specified

Request Quote

Description

Insect cells infected with high-titer baculovirus stock at a MOI=3, harvested 24 hours post infection, 5 vials cryopreserved at 1 x 10^7 cells/mL, stored in liquid nitrogen vapor phase

Deliverables

BIIC stocks delivered or stored at Proteos

Description

Baculovirus mediated expression of a secreted His-tagged protein in Tni PRO insect cells at the  20 L culture scale. Diafiltration of conditioned culture supernatant into a Ni-NTA compatible buffer. Single step Ni-NTA affinity purification, dialysis into destination buffer, concentration targeting 1 mg/mL. Delivered frozen on dry ice in 0.5 mL aliquots.

Deliverables

All purified protein and a data sheet with final results.

Required Materials & Information

Baculovirus transfer vector encoding a SECRETED His-tagged protein of interest, preferred MOI, destination buffer formulation

Additional Notes

Includes generation of high-titer baculovirus stock for infection, secreted protein expression only, TBS based destination buffer will be used if no destination buffer is specified, protein concentration will be terminated if precipitation is observed

SKUPP-008
TitlemAb or Fc-fusion protein production in HEK293 cells, 20 L scale
Quantity
Price$15,300.00
Addons

Base Pairs: +$( BasePairs * .40 )
Description

Production of synthetic cDNA with codon optimization for expression in E. coli, baculovirus, or mammalian cell (HEK293) expression systems. Subcloning of synthetic cDNA into appropriate expression or transfer vector for protein production.

Deliverables

Expression or transfer vector banked at Proteos for 1 year

Additional Notes

Vectors are not transferrable due to liscensing restrictions

Description

Endotoxin-free plasmid preparation at the giga-prep scale for mammalian cell expression (typically yields 10 mg of DNA)

Additional Notes

Can be added to HEK293 protein production packages. Must be high-copy number plasmid, must order one prep each for heavy chain and light chain if not using a dual expression vector

Description

Analytical size exclusion chromatography of purified protein to assess purity and monodispersity

Description

Size exclusion chromatography (gel filtration) following Protein A purification, 5x90 cm column, destination buffer of choice

Additional Notes

PBS destination buffer will be used if no destination buffer is specified

Request Quote

Description

Transient expression of mAb or Fc fusion protein in HEK293 cells at the 20 L culture scale.  Single step Protein A affinity purification, dialysis into into destination buffer,  concentration targeting 1 mg/mL. mAb delivered in a single tube on wet ice, Fc-fusion protein delivered frozen on dry ice in 0.5 mL aliquots.

Deliverables

All purified protein and a data sheet with final results.

Required Materials & Information

20 mg of endotoxin-free expression plasmid DNA, destination buffer formulation

Additional Notes

10 mg of each expression plasmid DNA encoding the heavy chain and light chain will be required if not  using a dual vector system, PBS will be used as destination buffer if no destination buffer is specified, maximum resin bed for Protein A will be 200 mL with a maximum of 2 passes

SKUPP-009
TitleMedium throughput recombinant mAb (IgG) production in HEK293 cells, 30 mL scale
Quantity
Price$19,000.00
Description

Transient expression of 48 recombinant mAb in parallel at the 30 mL culture scale. Single step Protein A affinity purification, dialysis into into destination buffer. Each mAb delivered in a single tube on wet ice.

Deliverables

All purified mAb and a data sheet with final results.

Required Materials & Information

Greater than 30 micrograms of endotoxin-free expression plasmid DNA for each mAb, destination buffer formulation

Additional Notes

Greater than 15 micrograms  of each expression plasmid DNA encoding the heavy chain and light chain will be required if not  using a dual vector system, PBS destination buffer will be used if no destination buffer is specified, purification will be via single pass Protein A affinity chromatography with a 150 microliter bed volume

SKUPP-010
TitleHigh throughput intracellular His-tagged protein production in E.coli, 15 mL scale
Quantity
Price$8,100.00
Description

96 constructs expressed at the 15 mL scale in a plate-based format, purified by Ni-NTA (single step), desalting step to remove imidizole from final elution pool, delivered frozen on dry ice

Deliverables

All purified protein and a data sheet with final results.

Required Materials & Information

Expression plasmid DNA  encoding proteins of interest or information regarding antibiotic resistance

Additional Notes

Up to 96 constructs/clones per plate, must be same antibiotic resistance for each plate

Recombinant Protein Products

SKU
Title
Quantity
Price
Purchase
SKURP-001
TitleHuman Alpha-synuclein monomer (1 mg)
Quantity
Price$225.00
Description

Full-Length human alpha-synuclein monomeric protein generated in vitro. This protein was used to generate alpha-synuclein filament which was then used as antigen for the MJF-14 alpha-synuclein filament-specific antibody available from Abcam (ab209538).

Deliverables

Protein is supplied in 1 mg aliquots with a COA

Required Materials & Information

Agree to terms and conditions upon checkout.

Additional Notes

10 x 1  mg aliquot maximum/order

SKURP-002
TitleHuman Alpha-synuclein filament
Quantity
Price$275.00
Description

Full-Length human alpha-synuclein protein expressed in vitro and manipulated to form alpha-synuclein filaments. This filament version of alpha-synuclein was used as antigen for the MJF-14 conformation-specific rabbit monoclonal alpha-synuclein filament specific antibody available from Abcam (ab209538).  

Deliverables

Protein is supplied in 1 mg aliquots with a COA

Required Materials & Information

Agree to terms and conditions upon checkout.

Additional Notes

10 x 1  mg aliquot maximum/order

SKURP-003
TitleHuman Alpha-synuclein monomer to generate preformed fibrils (Product is currently on backorder, earliest estimate for delivery is May 2018 )
Quantity
Price$225.00
Description

Full-Length human alpha-synuclein monomeric protein specially formulated to generate pre-formed fibrils. A protocol for generating pre-formed fibrils from this monomeric protein will be included with each order.  

Deliverables

Protein is supplied in 1 mg aliquots with a COA

Required Materials & Information

Agree to terms and conditions upon checkout.

Additional Notes

10 x 1  mg aliquot maximum/order

SKURP-004
TitleHuman Alpha-synuclein phospho-Ser129 peptide
Quantity
Price$325.00
Description

Phospho-S129 Alpha Synuclein semi-synthetic phosphomimetic peptide generated by native chemical ligation of a recombinant peptide produced from E. coli and a synthetic peptide.

Deliverables

Protein is supplied in 1 mg aliquots with a COA

Required Materials & Information

Agree to terms and conditions upon checkout.

Additional Notes

5 x 1  mg aliquot maximum/order

SKURP-005
TitleDJ-1 (Untagged, E. coli)
Quantity
PriceS&H
Description

Recombinant untagged human DJ-1 protein produced in E. coli. 

Deliverables

Protein is supplied in 1 mg aliquots with a COA

Required Materials & Information

Agree to terms and conditions upon checkout.

Additional Notes

2 x 1  mg aliquot maximum/order

SKURP-006
TitleDJ-1 (His-tagged, E. coli)
Quantity
PriceS&H
Description

Recombinant His-tagged human DJ-1 protein produced in E. coli.  

Deliverables

Protein is supplied in 1 mg aliquots with a COA

Required Materials & Information

Agree to terms and conditions upon checkout.

Additional Notes

2 x 1  mg aliquot maximum/order

SKURP-007
TitleDJ-1 (His-tagged, mammalian)
Quantity
PriceS&H
Description

Recombinant His-tagged DJ-1 protein produced in a mammalian cell expression system (HEK).

Deliverables

Protein is supplied in 1.6 mg aliquots with COA

Required Materials & Information

Agree to terms and conditions upon checkout.

Additional Notes

2 x 1  mg aliquot maximum/order

SKURP-008
TitleDJ-1 (TEV-cleaved, mammalian)
Quantity
PriceS&H
Description

Recombinant TEV-cleaved (to remove His-tag) DJ-1 protein produced in a mammalian cell expression system (HEK).

Deliverables

Protein is supplied in 1.05 mg aliquots with a data COA

Required Materials & Information

Agree to terms and conditions upon checkout.

Additional Notes

2 x 1  mg aliquot maximum/order

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