Chromatography Methods

Proteos offers fully customizable purification services for isolation of recombinant affinity-tagged or native proteins using manual and FPLC chromatography methods.  Purification scales range from less than a milligram up to multiple gram quantities at purities >95%.  Our scientists have decades of protein purification experience and can either develop a process from square one or replicate an established protocol.

Services Offered:

  • Classical (IEX, HIC, SEC, etc.) and affinity (IMAC, Protein A, FLAG, GST, etc.) chromatography for purification of recombinant proteins from E. coli, baculovirus infected insect cell, or mammalian cell expression systems, or customer provided biomass.
  • Purification of native proteins from BSL 1 biological samples
  • Manual or automated purification methods using AKTA FPLC
  • Large scale SEC capabilities for isolation of up to 10 grams of protein
  • Mechanical lysis capabilities from 10 mL to 2 L volumes via French press or microfluidizer
  • Concentration and diafiltration capabilities via tangential flow filtration
  • Membrane preparations
  • Endotoxin removal and low endotoxin protein preparation, useful for antigen production, cell based assays, and in-vivo studies
  • Formulation optimization - LEARN MORE
  • Protein labeling/conjugation (HRP, biotin, fluorescent labels, etc.)
  • Protein analysis - LEARN MORE

Multi-step Purification Gel Image

SDS-PAGE analysis of the enrichment observed through a three step purification scheme of an untagged recombinant protein.

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