Baculovirus Titer Assay
Proteos utilizes a robust economical plate-based flow cytometry assay for baculovirus titer determinations, allowing accurate optimization of infection parameters used for protein production in baculovirus infected insect cell cultures. Results can be obtained in as little as 3 business days from receipt of the samples, only requires 600 µL of baculovirus stock, and is performed in triplicate so you can be confident of the results. A full data sheet containing all of the assay data is included.
Thermal Shift Analysis
The thermal shift analysis (TSA) is a thermal denaturation assay for elucidating conditions that stabilize a protein in solution. Protein destabilization and melting is measured as an increase in fluorescence as hydrophobic binding sites within the protein become exposed during unfolding and are able to bind a fluorescent dye in the assay mixture. The melting temperature of a protein (Tm) is identified as the temperature at which 50% of the protein is unfolded. The assay is performed in plate format, allowing a large number of conditions (buffers, ligands, etc.) to be screened simultaneously. Conditions that cause the Tm to shift to a higher temperature are identified as stabilizing.
Custom Assay Services
Proteos offers custom assay services that can be designed to fit your specific project requirements. Additional available custom assay services include capillary cytometry, cell-based assays, ELISA, intact mass analysis (mass spectrometry), colorimetric and fluorometric enzymatic assays, protein assays, and LAL assay for endotoxin determination. Please contact us to discuss your custom assay needs.