Best Practices: Perform Small-Scale Expression Optimization
Proteos is an industry leader in the production of high-quality recombinant proteins for drug discovery research. There are many variables to consider when producing novel recombinant proteins. Here we will focus on the importance of performing small-scale expression optimization.
Small-Scale Pilot Experiments
Small-scale pilot experiments are the best way to determine the ideal expression conditions for a recombinant protein.
Comparing Systems
Bacterial Expression
For bacterial expression, screening different cell strains, growth mediums, and induction parameters, including temperature, duration, and inducer concentration can improve protein expression in E. coli.
Baculovirus-Infected Insect Cell Expression
For recombinant protein production in baculovirus-infected insect cells, small-scale expression optimizations can identify the best cell line, multiplicity of infection (MOI) and harvest time post-infection.
Mammalian Cell Expression
Similarly, pilot experiments conducted in mammalian cells can identify the ideal cell line, growth temperature, and harvest time based on cell viability.
Conclusions
No matter which expression system being employed, taking the time to optimize conditions during pilot production can drastically improve results during large-scale production.
This will conclude our informational blog series regarding best practices for novel recombinant protein production.
Get in Touch
The Proteos team knows that high quality protein reagents provide a solid foundation for successful, drug discovery. Contact Proteos today. We will guide you through the process and design a custom quotation that fits your specific project needs. Together, we can explore unique possibilities and overcome unexpected challenges to help you achieve your goals.
